MAKE ER RETENTION BASED DOMINANT NEGATIVE

General notes

This info comes from b.schwappach@zmbh.uni-heidelberg.de

Procedure

1. Use the ER retention signal KHILFRRRRRGFRQ and probably should put a little flexible linker like SSGSG in front. It has to be on the cytosolic side of the membrane and best on the C-terminus.

References

1. Schwappach, B., Zerangue, N., Jan, Y. N. and Jan, L. Y. (2000). Molecular basis for K(ATP) assembly: transmembrane interactions mediate association of a K+ channel with an ABC transporter. Neuron 26, 155-67.

This protocol is from Levin laboratory under Dr. Michael Levin at Tufts University.

PROTOCOL DEX