PROTOCOL DEX
About
Beta-Gal staining of Xenopus
Bovine fat and muscle differentiation
Bovine satellite cell (muscle) isolation
Bovine stromal vascular cell (fat) isolation
Cell harvest
Cell passage and sub culture
Cell thawing and design of experiment (DOE) preparation
Cell viability of antioxidant treated muscle cells
Chick floating filter culture
Chick in situ hybridization
Concentrating chick virus
Contact
Cryopreservation of cells
Design of experiment (DOE) conditions with Oil Red O assay and scaffold mechanical testing
Dialize sodium azide from antibody
Embryo vibratome sectioning
Make ER retention based dominant negative
Mammalian cell culture media preparation
Paralytic agents without ions
Paralyzing embryos with curare
Planaria care
Scaffold material preparation for lab grown meat
Slime trail visualization
Standardized nerve structure stain
TBARS assay for lipid oxidation
Thawing muscle cells and antioxidant treatments
Transcription of in situ probe
Verification of adipogenicity and myogenicity
Xenopus (frog) embryo stages collection
Xenopus egg preparation for injection
Xenopus lateral line visualization
Xenopus microinjection protocol
Xenopus transmembrane voltage tracker dye protocol
Xenopus whole mount immuno histochemistry protocol
PROTOCOL DEX